The superstar posted a video of his wife dancing as she sings Staying Alive.
Akshay Kumar, in his note for Twinkle Khanna, wrote that he loves her but she should stop singing.
"While you may be glad to have missed my live performance the other day, I'm glad I get to witness you and all your madness every single day! But as much as I love you, I really think you should stop singing And Happy birthday Tina," read his caption.
Twinkle Khanna and Akshay Kumar have been married for over 21 years.
They co-starred in films such as International Khiladi and Zulmi.
The couple is parents to 20-year-old son Aarav and daughter Nitara, 10.