Samson has introduced two changes to his side.
While Trent Boult replaces Kuldeep Yadav, KM Asif plays the match instead of Murugan Ashwin.
KKR captain Nitish Rana said Anukul Roy is set to replace Vaibhav Arora in his team for the match.
The match is vital for both RR and KKR to qualify for the playoffs.
RR and KKR are currently placed at fifth and sixth positions respectively in the points table.
The winner of the match will jump to the third spot disrupting the order of the IPL standings.
Rajasthan Royals XI: J Buttler, Y Jaiswal, S Samson (c & wk), J Root, S Hetmyer, D Jurel, Asif K M, R Ashwin, Y Chahal, T Boult, S Sharma.
Kolkata Knight Riders XI: J Roy, V Iyer, R Gurbaz (wk), S Narine, N Rana (c), R Singh, A Russell, S Thakur, A Roy, H Rana, V Chakaravarthy.